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Product Details
Synonyms
RANKL, CD254, TRANCE, OPGL, ODF
Source
Rhesus macaque TNFSF11 Protein, His Tag (RAL-R5243) is expressed from human 293 cells (HEK293). It contains AA Gly 136 - Asp 317 (Accession # F7F5R8).
Predicted N-terminus: Gly 136
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Rhesus macaque TNFSF11 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) at 1 μg/mL (100 μL/well) can bind Human Anti-TNFSF11, Human IgG1 with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Immobilized Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) at 1 μg/mL (100 μL/well) can bind Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
Immobilized Human Anti-TNFSF11, Human IgG1 at 1 μg/mL (100 μL/well) can bind Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 11
- Category：
- Listed Drugs Count：
18 Details
- Clinical Drugs Count：
18 Details
- Highest R&D Stage：
Approved
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