Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Nanodisc)

AIDD‑Ready
Related Products: Cell line CHEK-ATP154
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Menge
ST1-H5286-20ug
$1,440.00
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ST1-H5286-100ug
$5,355.00
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ST1-H5286-500ug
$12,920.00
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ST1-H5286
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Application
  • Cell based assay (FACS or functional assay)
  • Affinity testing (SPR / BLI)
  • Screening (ELISA)
Synonyms

STEAP1, PRSS24, STEAP

Source

Nanodisc Human STEAP1 Full Length Protein, Twin-Strep,His Tag (ST1-H5286) is expressed from human 293 cells (HEK293). It contains AA Glu 2 - Leu 339 (Accession # Q9UHE8-1).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a twin strep tag at the N-terminus and a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 44.7 kDa.

*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.

Structure

Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

Synthesis Process

Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.

Background Introduction

A transmembrane metalloreductase highly overexpressed in prostate cancer with minimal normal tissue distribution. It facilitates cellular iron and copper reduction. Its tumor-selective profile makes it a compelling target for antibody-drug conjugates and T-cell engagers in metastatic prostate cancer.