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Product Details
Synonyms
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
SARS coronavirus PC4-127 Spike Trimer Protein, His Tag (SPN-S52H0) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAU04664.1 (R667A, KV968-969PP)).
Predicted N-terminus: Ser 14
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 136.4 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
SARS coronavirus PC4-127 Spike Trimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized SARS coronavirus PC4-127 Spike Trimer Protein, His Tag (Cat. No. SPN-S52H0) at 5 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.008-0.5 μg/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Coronavirus spike glycoprotein
- Category：
- Listed Drugs Count：
15 Details
- Clinical Drugs Count：
88 Details
- Highest R&D Stage：
Approved
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