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Product Details
Synonyms
Prolactin R, PRLR, PRL-R
Source
Human Prolactin R, His Tag (PRR-H52Ha) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Asp 234 (Accession # P16471-1).
Predicted N-terminus: Gln 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.3 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Prolactin R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Prolactin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PRN-H5257) at 5 μg/mL (100 μL/well) can bind Human Prolactin R, His Tag (Cat. No. PRR-H52Ha) with a linear range of 20-156 ng/mL (QC tested).
Protocol
Immobilized Human GH, Tag Free at 5 μg/mL (100 μL/well) can bind Human Prolactin R, His Tag (Cat. No. PRR-H52Ha) with a linear range of 0.313-5 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Prolactin receptor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 3 Clinical
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