user
menu
letter
Sign InRegister
langEnglisharrow

Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag

Cat. No. / Size
Price
Availability
Qty
OV6-V81G9-25ug
$300.00
In Stock - Delivery in 1-2 business days
OV6-V81G9-200ug
$1,110.00
In Stock - Delivery in 1-2 business days
OV6-V81G9
Sub-Total :
$ 0
Customize Product Request Quote
Contact Us
Contact Us
Sub-Total:
$ 0
Request Quote
Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

OV16 (Onchocerca volvulus)

Source

Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag (OV6-V81G9) is expressed from E. coli cells. It contains AA Lys 17 - Asp 197 (Accession # P31729-1).

Predicted N-terminus: Met

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a GST tag.

The protein has a calculated MW of 48.8 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE).

The protein is designed as a dimer.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

The OV16 antigen, localizes to the hypodermis, cuticle, and uterus of female O. volvulus is regarded as a highly sensitive and specific antigen for detection of IgG4 in people with prior exposure to onchocerciasis. It has an open reading frame that encodes a 152- amino acid polypeptide containing a 16-amino acid putative signal peptide. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite.