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Human Lymphotoxin alpha1/beta2 Protein, His Tag (MALS verified)

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Product Number/Spec
Price
Menge
LY2-H5243-100ug
$350.00
LY2-H5243-1mg
$2310.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    Lymphotoxin alpha1/beta2

  • Source

    Human Lymphotoxin alpha1/beta2 Protein, His Tag (LY2-H5243) is expressed from human 293 cells (HEK293). It contains AA Lys 62 - Leu 205 (alpha1) & Asp 82 - Gly 244 (beta2) (Accession # P01374 (alpha1) & Q06643 (beta2)).

    Predicted N-terminus: His

  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 53.8 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    Lymphotoxin alpha1/beta2 SDS-PAGE

    Human Lymphotoxin alpha1/beta2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Lymphotoxin alpha1/beta2 SEC-MALS

    The purity of Human Lymphotoxin alpha1/beta2 Protein, His Tag (Cat. No. LY2-H5243) is more than 90% and the molecular weight of this protein is around 48-73 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Lymphotoxin alpha1/beta2 ELISA

    Immobilized Human LTBR, Fc Tag (Cat. No. LTR-H5259) at 5 μg/mL (100 μL/well) can bind Human Lymphotoxin alpha1/beta2 Protein, His Tag (Cat. No. LY2-H5243) with a linear range of 2-63 ng/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Lymphotoxin alpha1/bate2 (LT α1/β1) are members of the tumor necrosis factor (TNF) superfamily, and plays a pivotal role in the establishment and regulation of the immune system. The predominant form on the lymphocyte surface is the lymphotoxin alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin beta receptor (LTβR). The minor complex is lymphotoxin alpha 2/beta 1. Lymphotoxin alpha mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Lymphotoxin beta is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue.

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