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Product Details
Synonyms
Latent TGF-beta 1, Latent TGFB1, TGFB1, CED, DPD1, LAP, TGF-beta-1, TGFB
Source
Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (TG1-C5243) is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (C33S) (Accession # XP_005589396.1).
Predicted N-terminus: His & Leu 280
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus. The precursor proprotein is partially cleaved by FURIN to form mature Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains with calculated MW of 12.7 kDa and 30.4 kDa respectively. The protein migrates as 50-60 kDa (precursor proprotein), 38-45 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Cynomolgus Latent TGF-Beta 1 (C33S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-H82E4) with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT8-H82W5) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Transforming growth factor beta 1
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
22 Details
- Highest R&D Stage：
Approved
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