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Product Details
Synonyms
IL-6 R alpha & IL-6
Source
Human IL-6 R alpha & IL-6 Protein, His Tag (IL6-H52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 & Val 30 - Met 212 (Accession # P08887-1 & P05231-1).
Predicted N-terminus: Leu 20 & Val 30
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 65.0 kDa & 20.8 kDa. The protein migrates as 70-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human IL-6 R alpha & IL-6 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human IL-6 R alpha & IL-6 Protein, His Tag (Cat. No. IL6-H52H4) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human gp130 Protein, Fc Tag (Cat. No. ILT-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha & IL-6 Protein, His Tag (Cat. No. IL6-H52H4) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human gp130 Protein, Fc Tag (Cat. No. ILT-H5254) captured on Protein A Chip can bind Human IL-6 R alpha & IL-6 Protein, His Tag (Cat. No. IL6-H52H4) with an affinity constant of 0.036 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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