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Product Details
Synonyms
IL12, p70, Interleukin-12
Source
Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag (IL2-M52W3) is expressed from human 293 cells (HEK293). It contains AA Met 23 - Ser 335 (IL-12B) & Arg 23 - Ala 215 (IL-12A) (Accession # P43432 (IL-12B) & P43431 (IL-12A)).
Predicted N-terminus: Arg 23 & Met 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag, produced by co-expression of IL-12B and IL-12A, has a calculated MW of 35.8 kDa (IL-12B) and 23.6 kDa (IL-12A). Subunit IL-12B is no tag and subunit IL-12A contains a polyhistidine tag at the C-terminus. The protein migrates as 28-30 kDa, 42-43 kDa and 45-46 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag (Cat. No. IL2-M52W3) is more than 90% and the molecular weight of this protein is around 55-80 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag (Cat. No. IL2-M52W3) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-12 R beta 1, His,Avitag (Cat. No. ILB-M52E1) with a linear range of 0.3-20 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag (Cat. No. IL2-M52W3) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-12
- Category：
- Listed Drugs Count：
12 Details
- Clinical Drugs Count：
35 Details
- Highest R&D Stage：
Approved
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