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IGHG4
Human IgG4 Fc, Tag Free (IG4-H5205) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 327 (Accession # P01861).
Predicted N-terminus: Glu 99
This protein carries no "tag".
The protein has a calculated MW of 25.8 kDa. The protein migrates as 30-33 kDa under reducing (R) condition, and 29 kDa and 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.