Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (MALS & SPR verified)

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HNN-M52H3-50ug
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Synonyms

Hemagglutinin-neuraminidase/HN (MuV)

Source

Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (HNN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 106 - Thr 582 (Accession # P11235).

Predicted N-terminus: Gly 106

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 54.6 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

Mumps immunity is typically assessed by measuring neutralizing-antibody responses directed against mumps HN and F proteins.