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Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (MALS verified)

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Menge
H2A-M52C3-25ug
$290.00
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H2A-M52C3-200ug
$1,650.00
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H2A-M52C3
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Synonyms

H-2Kb & B2M & OVA (SIINFEKL)

Source

Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (H2A-M52C3) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Met 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide (Accession # P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL).

Predicted N-terminus: Gly 22 & Ser

Molecular Characterization

Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein is assembled by biotinylated monomer (H2A-M82E6) and streptavidin.

Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 39.3 kDa and 17.6 kDa and 13.8 kDa. The protein migrates as 50-52 kDa, 19 kDa and 14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background Introduction

Ovalbumin (OVA) can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. ovalbumin (Ova) was presented to T cells if it was released from pinosomes into the cytosol by osmotic lysis of pinosomes. Immunization with the adjuvanted SIINFEKL peptide induces long-lasting CD8+ T cell immunity in mice.