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Product Details
Synonyms
H-2Kb & B2M & OVA (SIINFEKL)
Source
Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (H2A-M52C3) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Met 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide (Accession # P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL).
Predicted N-terminus: Gly 22 & Ser
Molecular Characterization
Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein is assembled by biotinylated monomer (H2A-M82E6) and streptavidin.
Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 39.3 kDa and 17.6 kDa and 13.8 kDa. The protein migrates as 50-52 kDa, 19 kDa and 14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Cat. No. H2A-M52C3) is more than 90% and the molecular weight of this protein is around 295-320 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Cat. No. H2A-M52C3) at 1 μg/mL (100 μL/well) can bind InVivoMAb anti-mouse MHC Class I (H-2Kb) bound to SIINFEKL peptide (OVA residues 257-264) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Immobilized Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Cat. No. H2A-M52C3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-B2M Antibody, Human IgG1 with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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