Product Details
Synonyms
Glycoprotein B/gB (HSV-2)
Source
Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (GLB-V82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Ala 730 (Accession # P06763).
Predicted N-terminus: Glu 98
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 76.0 kDa. The protein migrates as 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (Cat. No. GLB-V82E3) is more than 90% and the molecular weight of this protein is around 235-270 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (Cat. No. GLB-V82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse anti-Herpes virus 1 and 2, clone T111, Mouse IgG1 with a linear range of 0.02-1 ng/mL (QC tested).
Protocol
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