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Product Details
Synonyms
GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8
Source
Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (GD8-C82Q3) is expressed from human 293 cells (HEK293). It contains AA Gly 19 - Ser 375 (Accession # Q95J86).
Predicted N-terminus: Gly
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 44.1 kDa. The protein migrates as 11-12 kDa, 40-43 kDa and 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Apitegromab with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) with a linear range of 0.002-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with a linear range of 0.5-16 ng/mL (Routinely tested).
Protocol
Immobilized Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Apitegromab captured on Protein A Chip can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with an affinity constant of 25.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Growth/differentiation factor 8
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
8 Details
- Highest R&D Stage：
NDA/BLA
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