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Product Details
Features and Advantages
- Seamless Transition from Preclinical to Clinical: Same cell clone, process, and QC as GMP products.
- Enhanced Production Standards: AOF raw materials, pharma-grade excipients, Grade B+A (ISO 5) environment.
- Comprehensive Quality Control: Additional strict testing for process impurities, sterility, and mycoplasma.
- Cost-Effective Solution: GMP-comparable quality at an economical price for early development.
- Risk Mitigation: Enhanced safety through rigorous impurity control and comprehensive testing.
Synonyms
FLT3LG, FL, FLT3L, Flt3 ligand
Source
Human Flt-3 Ligand Protein (E. coli), premium grade (FLL-H5115) is expressed from E. coli cells. It contains AA Thr 27 - Ala 181 (Accession # P49771-1).
Predicted N-terminus: Met
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
GMP-FLLH13 is the GMP version of this FLL-H5115. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stage to later clinical phases.Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 17.7 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Quality Description
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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Data Display
SDS-PAGE
Human Flt-3 Ligand Protein (E. coli), premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Flt-3 Ligand Protein (E. coli), premium grade (Cat. No. FLL-H5115) is more than 95% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.
Report
Bioactivity-CELL BASE
Human Flt-3 Ligand Protein (E. coli), premium grade (Cat. No. FLL-H5115) stimulates proliferation of OCI-AML5 cells. The specific activity of Human Flt-3 Ligand Protein (E. coli), premium grade is > 5.00 x 10^5 IU/mg, which is calibrated against human WHO-Flt3 Ligand International Standard (NIBSC code: 96/532) (QC tested).
Protocol
Application Data
Human hematopoietic stem and progenitor cells (HSPCs) were expanded ex vivo for 9 days using a cytokine cocktail containing GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Flt-3 Ligand Protein (E. coli) (Cat. No. GMP-FLLH13), and GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25). The cell growth curve and cell viability are analyzed by AO/PI staining. The results demonstrate that GMP Human Flt-3 Ligand Protein (E. coli) could support the rapid cell expansion and good cell viability of HSPCs, exhibiting performance comparable to that of Human Flt-3 Ligand Protein (E. coli), premium grade (Cat. No. FLL-H5115).
Human hematopoietic stem and progenitor cells (HSPCs) were cultured for 3 days in medium supplemented with GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH13), and GMP Human Thrombopoietin (TPO) Protein (Cat. No. GMP-THNH25). Flow cytometry was used to assess the expression of surface markers CD34 and CD45. The results demonstrate that GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH13) promoted HSPC proliferation to a similar extent as Human Flt-3 Ligand Protein (E. coli), premium grade (Cat. No. FLL-H5115).
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Fms-related tyrosine kinase 3 ligand
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Phase 2 Clinical
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