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Product Details
Synonyms
Fc Receptor Blocking Solution
Source
Human Fc Receptor Blocking Solution Protein, Tag Free (FCN-H5213) is expressed from human 293 cells (HEK293).
Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 31.2 kDa.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Data Display
Bioactivity-FACS
Flow cytometric analysis was performed on THP-1 cells that were either treated with Human Fc Receptor Blocking Solution Protein, Tag Free (Cat. No. FCN-H5213) using a 1:20 dilution (5 μL of antibody stock per 1e6 cells in a final volume of 100 µL) or left untreated. All cells were stained with an irrelevant PE-conjugated mouse IgG2a antibody. PE signal was used to evaluate the blocking effect (QC tested).
Protocol
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