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Product Details
Synonyms
FCGR1A, FCG1, FCGR1, IGFR1, CD64, CD64A, FCRI
Source
Biotinylated Cynomolgus CD64, His,Avitag (FCA-C82E8) is expressed from human 293 cells (HEK293). It contains AA Val 11 - Pro 288 (Accession # NP_001270969.1).
Predicted N-terminus: Val 11
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 34.8 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Cynomolgus CD64, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Cynomolgus CD64, His,Avitag (Cat. No. FCA-C82E8) is more than 90% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Ipilimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD64, His,Avitag (Cat. No. FCA-C82E8) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized FMC63 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) (Cat. No. FCA-C82E8) with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
Immobilized Cynomolgus IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M553) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD64, His,Avitag (Cat. No. FCA-C82E8) with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Cynomolgus CD64, His,Avitag (Cat. No. FCA-C82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin with an affinity constant of 1.50 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Cynomolgus CD64, His,Avitag (Cat. No.FCA-C82E8) on SA Biosensor, can bind Herceptin with an affinity constant of 0.326 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
User Reviews Publish Comment
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
Frontier Progress
Drug Development Progress- English Name：
High affinity immunoglobulin gamma Fc receptor I
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Preclinical
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