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Product Details
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Rhesus macaque EGF R, Fc Tag (EGR-C5252) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P55245).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 95.3 kDa. The protein migrates as 116-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Rhesus macaque EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rhesus macaque EGF R, Fc Tag (Cat. No. EGR-C5252) is more than 85% and the molecular weight of this protein is around 225-245 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human EGF, Tag Free at 10 μg/mL (100 μL/well) can bind Rhesus macaque EGF R, Fc Tag (Cat. No. EGR-C5252) with a linear range of 39-1250 ng/mL (QC tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Rhesus macaque EGF R, Fc Tag (Cat. No. EGR-C5252) at 10 μg/mL, add increasing concentrations of Amivantamab and then add Cynomolgus HGF R, His Tag (Cat. No. MET-C52H9) at 5 μg/mL. Detection was performed using HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with sensitivity of 0.02 μg/mL (Routinely tested).
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Epidermal growth factor receptor erbB1
- Category：
- Listed Drugs Count：
39 Details
- Clinical Drugs Count：
206 Details
- Highest R&D Stage：
Approved
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