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CNR1, cannabinoid receptor 1, CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR
Detergent Biotinylated Human CNR1 Protein, Flag Tag&His,Avitag (CN1-H85D3) is expressed from Baculovirus-Insect cells. It contains AA Gly 99 - Ala 301 & Asp 333 - Ser 414 (Accession # P21554-1).
Predicted N-terminus: Asp
This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™).
The protein has a calculated MW of 60.3 kDa. The protein migrates as 43-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Within the expressed region, several mutations were introduced to optimize the protein expression and stability.
*The detergent Buffer B (Cat. No. LG-13) is sold separately and not included in protein, you can follow the link for product information.
Each full-length transmembrane protein is encapsulated by detergent micelle.
Through mild detergent micelle extraction, dedicated solubilization, and stringent affinity purification, we maximally retain the native transmembrane conformation of full-length transmembrane proteins throughout downstream purification. This workflow yields purified, detergent-solubilized proteins free of unrelated membrane impurities and exogenous scaffold proteins, with exceptional purity, accurate quantitation, and well-preserved biological activity—ideal for immunization, binding assays in antibody discovery and screening (ELISA) and affinity testing (SPR and BLI), etc.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
>85% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of detergent.
Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove detergent.
Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer B, pH7.5, CHS with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.