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Claudin-6, CLDN6
Nanodisc Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (CL6-HF2M9) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Val 220 (Accession # P56747).
Predicted N-terminus: Met
This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus.
The protein has a calculated MW of 54.0 kDa. The protein migrates as 22 kDa, 25 kDa, 51-52 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
GFP
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 530 nm
>80% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped on dry ice.
Please protect from light and avoid repeated freeze-thaw cycles.