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CD8 alpha & beta, CD8A & CD8B
Biotinylated Cynomolgus CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free (CDA-C82W3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 & Leu 22 - Pro 170 (Accession # XP_065382910.1 & XP_005575417.1).
Predicted N-terminus: Ser 22 & Leu 22
Biotinylated Cynomolgus CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 25.4 kDa (CD8 alpha) and 21.1 kDa (CD8 beta). Subunit CD8 alpha is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit CD8 beta contains no tag at the C-terminus. The protein migrates as 30-32 kDa and 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.