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Product Details
Synonyms
CD5, LEU1
Source
Alexa Fluor 647-Labeled Human CD5 Protein, His Tag (CD5-HA2H7) is produced via conjugation of AF647 to Human CD5 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD5 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Pro 372 (Accession # P06127-1).
Predicted N-terminus: Arg 25
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Protein Ratio
The AF647 to protein molar ratio is 0.65-1.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
Bioactivity-ELISA
Immobilized Alexa Fluor 647-Labeled Human CD5 Protein, His Tag (Cat. No. CD5-HA2H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD5 Antibody, Mouse IgG1 (2C8A7) with a linear range of 0.06-4 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
Bioactivity-FACS
1e5 of Mouse Anti-CD5 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD5 Protein, His Tag (Cat. No. CD5-HA2H7) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD5 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD5 Protein, His Tag (Cat. No. CD5-HA2H7) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
T-cell surface glycoprotein CD5
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
13 Details
- Highest R&D Stage：
Phase 2 Clinical
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