Alkaline phosphatase (4U/ul)

Alkaline Phosphatases (APs) is derived from Antarctic bacterium and expressed in E. coli. APs nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. Also, APs Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs).

Dephosphorylation 5´ and 3´ ends of DNA and RNA.
Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.
Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase.
Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis.

One unit is defined as the amount of enzyme that will dephosphorylate 1 µg of pUC19 vector DNA cut with a restriction enzyme generating 5´ recessed ends in 30 minutes at 37°C.

>90% as determined by SDS-PAGE.

Alkaline Phosphatase is inhibited by metal chelators (e.g. EDTA), inorganic phosphate and phosphate analogs.

Alkaline Phosphatase activity is decreased in the presence of reducing agents (DTT, β-ME).

Supplied as 0.2 μm filtered solution in 10 mM Tris, pH7.4 with glycerol as protectant.

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This product is supplied and shipped on dry ice.

• The product MUST be stored at -20°C or lower upon receipt.
• -20°C for 12 months under sterile conditions.