HRV-3C Protease, His Tag (10U/μL)

HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with polyhistidine tags, can be easily removed after cleavage.

Removal of purification tags from fusion proteins.

One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.

>90% as determined by SDS-PAGE.

>10 U/μL

Supplied as 0.2 μm filtered solution in 25 mM Tris, 150 mM NaCl with glycerol as protectant.

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This product is supplied and shipped on dry ice.

• The product MUST be stored at -70°C or lower upon receipt;
• -70°C for 12 months under sterile conditions.