Influenza A (H3N2) Viruses Neuraminidase (NA) Specific ELISA Kit

Influenza A (H3N2) Viruses Neuraminidase () Specific ELISA Kit is designed for quantitative detection of antigen and quality control of vaccines.It is based on sandwich-ELISA technology platform to realize the quantitative detection of protein in samples.

This kit is developed for specific quantitative detection of Influenza A (H3N2) viruses Neuraminidase (NA) in samples.

It is for research use only.

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Influenza A (H3N2) viruses Neuraminidase (NA) protein. The kit consists of Pre-coated Anti-NA (Influenza A (H3N2)) Antibody Microplate and NA (Influenza A (H3N2)) Standard,HRP-Anti-NA (Influenza A (H3N2)) Antibody and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add your sample to the plate, take the NA (Influenza A (H3N2)) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add the HRP-Anti-NA (H3N2) Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.