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HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb

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IGG-LY69-100ug
$360.00
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IGG-LY69-1mg
$2,380.00
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IGG-LY69
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Source

HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Cat. No. IGG-LY69) is a mouse monoclonal antibody produced from a hybridoma generated by fusion of SP2/0 myeloma cells with mouse B lymphocytes.

Antibody Type

Hybridoma Monoclonal

Clone

6F11C8

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

Human

Immunogen

Human-IgG-Fc

Conjugate

HRP conjugated

Specificity

This product can react with the Fc portion of Human IgG1/ IgG2/ IgG3/ IgG4 and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A/L235A), Human IgG1 (M252Y/S254T/T256E), Human IgG1 (L234F/L235E/P331S), Human IgG1 (S239D/I332E), Human IgG1 (S298A/E333A/K334A), Human IgG1 (T307A/E380A/N434A), Human IgG2 (A330S/P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P/L235E).

Application
Application
Recommended Use
ELISA
1:800-1:8000

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Cross Verification

This antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, and IgG4) and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A/L235A), Human IgG1 (M252Y/S254T/T256E), Human IgG1 (L234F/L235E/P331S), Human IgG1 (S239D/I332E), Human IgG1 (S298A/E333A/K334A), Human IgG1 (T307A/E380A/N434A), Human IgG2 (A330S/P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P/L235E).
Weak cross-reactivity with cynomolgus IgG was observed by ELISA, whereas no reactivity to mouse IgG isotypes (IgG1, IgG2a, IgG2b) was detected.

Purification

Protein A purified.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 6 months after reconstitution;
  • 2-8°C for 2-3 weeks under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

ACRO Quality Management System

Background

Immunoglobulins can be divided into five main classes/isotypes which are IgA, IgD, IgE, IgG, and IgM. IgG class identity is determined by class-specific sequences in the Fc region of the heavy chain. IgG antibody class are the most abundant immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4). The IgG subclasses differ in their physical and chemical properties. Their distribution pattern is found to be age dependent and every subclass has a specific biological function.