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Organs-on-Chips

Organs-on-Chips are advanced in vitro model systems that integrate microfluidic technology with cell biology. By culturing cells within micro-scale channels and chambers, these chips can precisely replicate the microenvironment, physiological functions, and dynamic behaviors of cells. Typically made from transparent, biocompatible materials, Organs-on-Chips allow for real-time imaging and dynamic regulation. Compared to traditional 2D cell cultures and animal models, they offer a more physiologically relevant platform for drug screening, toxicity testing, and disease modeling. As the biopharmaceutical industry increasingly demands high-throughput and high-precision preclinical testing, Organs-on-Chips are emerging as a key technology to enhance drug development efficiency, reduce costs, and improve clinical translation success rates.

Through Acro Certify, ACROBiosystems has partnered with Netri to provide standardized, high-quality Organs-on-Chips products, along with comprehensive training and expert technical support. This enables efficient in vitro modeling and functional research, helping accelerate the translation of scientific discoveries into clinical applications.

Organs-on-Chips Products

NeuroFluidics™
NeuroFluidic MEA™
MultiFluidics™
Organs-on-Chips Starter Kits
Training and Technical Support

NeuroFluidics™

NeuroFluidics™ is an Organs-on-Chips platform specifically designed for neuroscience research. By integrating microfluidic channels with neural cell culture technology, it enables the in vitro simulation of brain physiology and neural circuitry. The platform adopts the standardized NeoBento format, capable of holding up to four QuarterBentos, which allows for a maximum of 16 chips. NeoBento supports various chip combinations and can be configured in either a LIGHT or FULL version, depending on experimental needs. Additionally, the platform is compatible with multiple chip types, including DuaLink, DuaLink Shift, and TriaLink, offering flexible options to meet diverse research requirements.

DuaLink

For 2 co-cultures

DuaLink Shift

For synaptic isolation

TriaLink

For 3 co-cultures

Key Features:

Microfluidic Compartmentalization: Enables spatial segregation of neuronal subtypes or regions, supporting the formation of directional neural networks.
Standardized High-Throughput Format: NeoBento adopts a standard ANSI format, ensuring broad compatibility and easy handling, just like a 96-well plate.
Compatible with Various Neural Cell Types: Suitable for primary neurons, iPSC-derived neurons, microglia, and co-culture systems to meet diverse experimental needs.
Supports Complex Neural Circuit Modeling: Designed to guide axon growth, promote synapse formation, and facilitate signal transmission for functional neural network reconstruction.
Ideal for Degenerative Disease Research: Enables long-term culture and neural injury modeling to study neuroinflammation and degenerative pathology.

NeuroFluidics™

DuaLink

For 2 co-cultures

DuaLink Shift

For synaptic isolation

TriaLink

For 3 co-cultures

Key Features:

NeuroFluidics™

DuaLink

For 2 co-cultures

DuaLink Shift

For synaptic isolation

TriaLink

For 3 co-cultures

Key Features:

NeuroFluidics™

DuaLink

For 2 co-cultures

DuaLink Shift

For synaptic isolation

TriaLink

For 3 co-cultures

Key Features:

NeuroFluidics™

DuaLink

For 2 co-cultures

DuaLink Shift

For synaptic isolation

TriaLink

For 3 co-cultures

Key Features:

Product List

Case Study

E18 rat embryo-derived hippocampal neurons were seeded into Culture Channel 1 of NeoBento™, while cortical neurons were seeded into Culture Channel 3, establishing an in vitro co-culture system. The channels were pre-coated with poly-D-lysine (PDL) and laminin to promote neuron adhesion, growth, and the gradual maturation of neural networks. The culture process lasted for 21 days, with the culture medium changed three times a week to maintain an optimal growth environment.

To assess the neuronal state and maturity, β-III-tubulin and DAPI were used for visualizing cell morphology, and MAP2 was employed to label neuronal dendrites to evaluate their maturation. Additionally, electrophysiological recordings using MEA were performed on days 7, 14, and 21 to measure spontaneous neuronal firing activity.

A. Immunofluorescence staining images of rat hippocampal neurons (Culture Channel 1) and cortical neurons (Culture Channel 3). Neurons successfully attached to the culture channels and extended axons. DAPI stains nuclei (blue), β-III-tubulin marks cell bodies and axons (green), and MAP2 labels dendrites (red). Scale bar = 500 µm.
B. Brightfield image of the rat neuron co-culture system on day 15, showing clear axonal extension.
C. Raster plots of the co-culture system on days 7, 14, and 21 (recording duration: 300 seconds). Synchronized firing activity began on day 14 and became more pronounced by day 21, indicating gradual network maturation.
D. Comparison of the mean firing rate (MFR) between the culture channels and connecting microchannel on days 7, 14, and 21. MFR increased significantly over time. On day 14, higher spontaneous activity (0.8 spikes/s) was observed in the microchannel, compared to 0.3 spikes/s in the culture channels, suggesting faster functional development of axonal pathways in the microchannel.