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| Assay Type | Sandwich-ELISA |
| Analyte | GM-CSF |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | 10.0 ng/mL |
| Standard Curve Range | 12.5 ng/mL-1600 ng/mL |
| Assay Time | 2 hr |
| Suitable Sample Type | For the quantitative determination of human GM-CSF in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| ID | Components | Size |
| CRB007-C01 | Pre-coated Anti-GM-CSF Antibody Microplate | 1 plate |
| CRB007-C02 | Human GM-CSF Standard | 32 μg×2 |
| CRB007-C03 | Biotin-Anti-GM-CSF Antibody Con. Solution | 100 μL |
| CRB007-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CRB007-C05 | Streptavidin-HRP Con. Solution | 500μL |
| CRB007-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CRB007-C07 | 20× Washing Buffer | 50 mL |
| CRB007-C08 | 1× Dilution Buffer | 15 mL×2 |
| CRB007-C09 | Substrate Solution | 12 mL |
| CRB007-C10 | Stop Solution | 6 mL |
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of GM-CSF in human serum and cell culture supernates.
It is for research use only.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of GM-CSF is less than 10.0 pg/mL.
Ten replicates of each of 3 samples containing different GM-CSF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of GM-CSF were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant GM-CSF was spiked into 5 human serum samples, and then analyzed. The average recovery of GM-CSF for serum samples is 102.82%.
Price(EUR) : €500.00
Price(EUR) :
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